Journal: Biology Open

Article Title: The neurotrophin DNT-2 via the Toll-2 receptor regulates neuronal survival and morphology during visual system development

doi: 10.1242/bio.062837

Figure Lengend Snippet: Identification of DNT-2 - and Toll-2 -expressing neurons. (A–C) DNT-2>mCherry neurons visualised with anti-DsRed antibodies colocalise the Mi1 marker anti-Bsh (arrowheads) at higher magnification in C. (D,E) Nuclei of Toll-2>Histone-YFP neurons colocalise with the L1 marker anti-Svp (arrowheads) at higher magnification in E. (F,G) Toll-2>hisYFP neurons colocalised with the anti-Erm L3 lamina neuron marker (arrowheads), at higher magnification in G. Stage: all 48 h APF. Scale bars: (A,B,D,F) 50 µm; (C,E,G) 20 µm.

Article Snippet: Primary antibodies used were as follows: mouse anti-24B10 (DSHB, Iowa) at 1:250 dilution; rabbit anti-GFP (Thermo Fisher Scientific) at 1:250 for normal stainings; mouse GRASP-specific anti-GFP (Sigma/Merck G6539) at 1:200; rabbit anti-Dcp1 (Cell Signalling) at 1:250; rat anti-N-cadherin (MAb DN-Ex) at 1:250; chicken anti-HA (Aves) at 1:100; rabbit anti-DsRed (Clontech, 632496) at 1:100; mouse anti-Svp (DSHB, Iowa) at 1:10; rabbit anti-Erm (a kind gift from Uwe Walldorf) at 1:100; guinea pig anti-Bsh (a kind gift from Larry Zipursky) at 1:500; and mouse anti-mCherry (DSHB, Iowa) at 1:50.

Techniques: Expressing, Marker

Journal: Bioactive Materials

Article Title: Smart microenvironment-adaptive nanocatalytic hydrogel for sequential antibacterial, anti-inflammatory, and regenerative therapy of biofilm-infected wounds

doi: 10.1016/j.bioactmat.2026.02.043

Figure Lengend Snippet: Characterization, and Cytocompatibility Validation of HCOC. (A) Schematic illustration of the development of HCOC. (B) FTIR spectrum of OSA, CMCS and OC hydrogel. (C) Time-dependent evolution of gelation of OC and HCOC. (D) SEM images of HCOC and EDS mapping images of C, N, O and Cu for HCOC. (E) FTIR spectra of HC, OC and HCOC. (F) Dynamic frequency sweep measurements of OC and HCOC. (G) Frequency-dependent viscoelastic behavior of OC and HCOC. (H) Alternating strain sweep with alternating strains of 1% and 1000% at 100s intervals and (I) Self-healing behavior of HCOC. (J) Live/dead staining showing the metabolic activity of L929 and RAW 264.7 cells after treatment with HCOC for 48 h. Rates of proliferation of (K) L929 cells and (L) RAW 264.7 cells after treatment with PBS or HCOC. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).

Article Snippet: Following the protocol of the DMAO/PI Bacterial Live/Dead Staining Kit (Beyotime Biotechnology), the bacteria were incubated with a working solution containing both DMAO and PI dyes in the dark at room temperature for 15-20 min. Fluorescence microscopy imaging was subsequently carried out.

Techniques: Biomarker Discovery, Staining, Activity Assay

Journal: Bioactive Materials

Article Title: Smart microenvironment-adaptive nanocatalytic hydrogel for sequential antibacterial, anti-inflammatory, and regenerative therapy of biofilm-infected wounds

doi: 10.1016/j.bioactmat.2026.02.043

Figure Lengend Snippet: pH Self-Adaptive Antioxidant Capacity of HCOC (Stage II: anti-inflammation). Cu ion release behavior of (A) HC (1 mg/mL) and (B) HCOC (1 mg/mL) at different pH levels. (C) ABTS + and (D) H 2 O 2 scavenging activity at different pH of Cu 5.4 O, HC and HCOC. (E) O 2 ∙ - , (F)∙OH scavenging activity of Cu 5.4 O, HAs, HC, HCOC. (G) SOD-like, (H) CAT-like and (I) GPx-like activities of HCOC. (J) Fluorescence images showing intracellular ROS detection by DCFH-DA staining, live/dead staining images and (K) cell viability of L929 cells with different treatments (All groups received 500 μM H 2 O 2 and different HCOC concentrations (I: PBS; II: 0; III: 0.25; IV: 0.50; V: 1.0 mg/mL HCOC). (L) Quantitative analysis of the cells under different treatments. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001) (M) Schematic illustration of pH-responsive activity and ROS scavenging and alleviating cellular oxidative stress of HCOC.

Article Snippet: Following the protocol of the DMAO/PI Bacterial Live/Dead Staining Kit (Beyotime Biotechnology), the bacteria were incubated with a working solution containing both DMAO and PI dyes in the dark at room temperature for 15-20 min. Fluorescence microscopy imaging was subsequently carried out.

Techniques: Activity Assay, Fluorescence, Staining